| Gene | Gp1bb |
| Chromosome | 16 |
| Deletion Start | 18,621,363 |
| Deletion End | 18,620,706 |
| Deletion Size | 658 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,621,298 | 18,621,317 | TGAGCCTGCTGCTTCTGTTG |
| TUP | 18,621,276 | 18,621,297 | CTAGCGCTGCTCAGCCGCCCAG |
| TUR | 18,621,226 | 18,621,244 | ACAGTCCACGAGCGTCCCT |
| TDF | 18,620,839 | 18,620,857 | GCTACACGCGCTGCTATTG |
| TDP | 18,620,814 | 18,620,837 | CACTTCTACTAGGTCGCCTGCGGA |
| TDR | 18,620,760 | 18,620,781 | GGGCTGTGAGAGAGAACTCTTG |
PCR Primers
| Primer | Sequence |
| SU | GCAGAATTGTGGTCTGTTGC |
| SD | AGACAGCTGGGTACAGGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12532A-A5 | VGB6 | C57BL/6NTac | |
| 12532A-B10 | VGB6 | C57BL/6NTac | |
| 12532A-E4 | VGB6 | C57BL/6NTac | |
| 12532A-E8 | VGB6 | C57BL/6NTac | |
