| Gene | C1qtnf5 |
| Chromosome | 9 |
| Deletion Start | 44,107,761 |
| Deletion End | 44,108,850 |
| Deletion Size | 1,090 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,108,156 | 44,108,178 | CAGAAACCCTGATGCCTCTACTC |
| TUP | 44,108,197 | 44,108,219 | CCCACAGGAGGCAATAGCCAGGG |
| TUR | 44,108,254 | 44,108,276 | GGAGAAATTAGGAGCCGCAGAAG |
| TDF | 44,108,707 | 44,108,726 | GGGCGTGGGTGATTACATTG |
| TDP | 44,108,730 | 44,108,758 | TCTATGCCAGCATCAAGACAGACAGTACC |
| TDR | 44,108,776 | 44,108,797 | GGAGCTGTGCCAGTCAGAATAG |
PCR Primers
| Primer | Sequence |
| SU | CTGAGGAAGCCATTCAAAGC |
| SD | GGAGCAGCAGAGATGGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12534A-D8 | VGB6 | C57BL/6NTac | |
| 12534A-G8 | VGB6 | C57BL/6NTac | |
| 12534A-H4 | VGB6 | C57BL/6NTac | |
| 12534A-H11 | VGB6 | C57BL/6NTac | |
