| Gene | Ccl11 |
| Chromosome | 11 |
| Deletion Start | 82,057,970 |
| Deletion End | 82,062,386 |
| Deletion Size | 4,417 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,057,995 | 82,058,013 | TGCTGCTCACGGTCACTTC |
| TUP | 82,058,015 | 82,058,036 | TTCACCTCCCAGGTGCTGGCTC |
| TUR | 82,058,054 | 82,058,077 | TGCCTGGCTTTCCTCAAAGATTAG |
| TDF | 82,061,928 | 82,061,949 | GAATTGTAACCCAGCCCGTGAG |
| TDP | 82,061,952 | 82,061,980 | TTGACGGCACATGTTTATTCCCAGATCTC |
| TDR | 82,061,983 | 82,062,002 | GCCAGGAACCAGCTCTGAAG |
PCR Primers
| Primer | Sequence |
| SU | ACCAGGTGAACAGGAACCAG |
| SD | GTGCAAAACAATAGACATTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12543A-B5 | VGB6 | C57BL/6NTac | |
| 12543A-A6 | VGB6 | C57BL/6NTac | |
