| Gene | Lilrb3 |
| Chromosome | 7 |
| Deletion Start | 3,720,323 |
| Deletion End | 3,712,639 |
| Deletion Size | 7,685 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,719,455 | 3,719,475 | CACCCAGCCTTTTAGCCCAAG |
| TUP | 3,719,429 | 3,719,453 | CAGCCCTGTGGTAACTTCAGGAGGG |
| TUR | 3,719,332 | 3,719,352 | ACTGTGAGTCTTGTGTCCACG |
| TDF | 3,713,587 | 3,713,610 | TCTTTAGCATGTGTCTGGTGATTC |
| TDP | 3,713,552 | 3,713,577 | ATATCCTGGATGGAGACGCCCACAGA |
| TDR | 3,713,526 | 3,713,546 | CCAGGAGTTGCTGTCCCTTAG |
PCR Primers
| Primer | Sequence |
| SU | CACAGGTGAGGCAGACACTG |
| SD | CTGGCCTTTGGTGGCTAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 144 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12545A-C2 | VGB6 | C57BL/6NTac | |
| 12545C-B10 | VGB6 | C57BL/6NTac | |
| 12545C-E1 | VGB6 | C57BL/6NTac | |
