| Gene | Pnpla2 |
| Chromosome | 7 |
| Deletion Start | 141,455,290 |
| Deletion End | 141,459,745 |
| Deletion Size | 4,456 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,455,749 | 141,455,771 | TCTTGGCTCATGGCATTCTCTTG |
| TUP | 141,455,773 | 141,455,793 | TGCCCATGTAGCCCGCCTTAA |
| TUR | 141,455,846 | 141,455,865 | TCCGCCCACGATTTACTTTC |
| TDF | 141,459,153 | 141,459,173 | TGCTCCCTTCTCAGCTTGTTG |
| TDP | 141,459,175 | 141,459,199 | AGTGGCTGCCTGATGTCCCTGAAGA |
| TDR | 141,459,209 | 141,459,230 | GCTACCCGTCTGCTCTTTCATC |
PCR Primers
| Primer | Sequence |
| SU | TTGCACTTCTCCATGTGCTC |
| SD | CCAGTTCACTGGGAGAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12546A-D6 | VGB6 | C57BL/6NTac | |
| 12546A-D10 | VGB6 | C57BL/6NTac | |
| 12546A-G2 | VGB6 | C57BL/6NTac | |
| 12546A-H9 | VGB6 | C57BL/6NTac | |
