| Gene | Efna4 |
| Chromosome | 3 |
| Deletion Start | 89,337,947 |
| Deletion End | 89,334,286 |
| Deletion Size | 3,662 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,337,358 | 89,337,377 | TGGCCCTGTTCTTCCATCTG |
| TUP | 89,337,331 | 89,337,354 | CACCTGTGGGCCAGTCATTTGACC |
| TUR | 89,337,265 | 89,337,285 | CACTTGCTTCCCAGGGTACTG |
| TDF | 89,334,788 | 89,334,808 | AGGTGTCTGTCTGCTGCAAGG |
| TDP | 89,334,761 | 89,334,785 | AGCGGTAAGTGAACCAGGGTCTCCC |
| TDR | 89,334,738 | 89,334,758 | CACACTGGCTGGAAGGACTAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCAGCACAGTGAGATAAC |
| SD | GCCCTCAGTGTCTCAAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12547A-C6 | VGB6 | C57BL/6NTac | |
| 12547A-C9 | VGB6 | C57BL/6NTac | |
| 12547A-D7 | VGB6 | C57BL/6NTac | |
| 12547A-D9 | VGB6 | C57BL/6NTac | |
| 12547A-F6 | VGB6 | C57BL/6NTac | |
