| Gene | Gm11808 |
| Chromosome | 4 |
| Deletion Start | 3,973,557 |
| Deletion End | 3,972,968 |
| Deletion Size | 590 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,973,025 | 3,973,045 | AGCCTGGTGAATGGCAGTTAG |
| TUP | 3,972,999 | 3,973,021 | CGCTGGCTGGGAAGACTCACTGG |
| TUR | 3,972,976 | 3,972,998 | CACAACTCCCTCCTCACAGTCTT |
| TDF | 3,973,019 | 3,973,039 | GTGAATGGCAGTTAGAAACGC |
| TDP | 3,972,995 | 3,973,018 | TGGCTGGGAAGACTCACTGGAAGA |
| TDR | 3,972,970 | 3,972,990 | TTGAACCACAACTCCCTCCTC |
PCR Primers
| Primer | Sequence |
| SU | ATACCCTTGGGTAACCAGCC |
| SD | TTCTTCATGTAGCATGTAGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12548A-A10 | VGB6 | C57BL/6NTac | |
| 12548A-H10 | VGB6 | C57BL/6NTac | |
| 12548A-G8 | VGB6 | C57BL/6NTac | |
| 12548A-E10 | VGB6 | C57BL/6NTac | |
| 12548A-C2 | VGB6 | C57BL/6NTac | |
| 12548A-D8 | VGB6 | C57BL/6NTac | |
