| Gene | Zfp467 |
| Chromosome | 6 |
| Deletion Start | 48,443,442 |
| Deletion End | 48,427,687 |
| Deletion Size | 15,756 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,442,734 | 48,442,756 | TCCCTCTGCCTATAACAGGATTC |
| TUP | 48,442,708 | 48,442,729 | TGGGACAGCCAGAGATGGCTCC |
| TUR | 48,442,660 | 48,442,681 | GACATCTTCTCCTGGGCATTAC |
| TDF | 48,428,221 | 48,428,240 | AGGGCATGATGAGCCCACAG |
| TDP | 48,428,196 | 48,428,218 | TGGTAGCACAGGCGCTGTTTACA |
| TDR | 48,428,162 | 48,428,182 | TGGTAGGCACAGGTCACATTG |
PCR Primers
| Primer | Sequence |
| SU | CACGTGTAGCTTCCCTCTCC |
| SD | AGCTCCCTGCTGAGACTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12550A-C8 | VGB6 | C57BL/6NTac | |
| 12550A-D4 | VGB6 | C57BL/6NTac | |
| 12550A-F8 | VGB6 | C57BL/6NTac | |
