| Gene | Higd1b |
| Chromosome | 11 |
| Deletion Start | 102,836,666 |
| Deletion End | 102,838,030 |
| Deletion Size | 1,365 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,837,206 | 102,837,224 | GCCCAGTGATCGCATTCTC |
| TUP | 102,837,225 | 102,837,245 | TGCCCACAGGTGTAGCAGGCT |
| TUR | 102,837,268 | 102,837,288 | CGAGCCTTCAGCCGGTAAATC |
| TDF | 102,837,513 | 102,837,534 | CTTGGTTCCTCATTGGTCACTG |
| TDP | 102,837,538 | 102,837,563 | CTGGGCCATGGGACAGTATGAAGACA |
| TDR | 102,837,589 | 102,837,608 | GCTTCTTCCGGGTTCTCATC |
PCR Primers
| Primer | Sequence |
| SU | AAAGACTGTTCCAGTCTACTC |
| SD | GTGGAAGGAATGCCTGTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12555A-B7 | VGB6 | C57BL/6NTac | |
