| Gene | Tff2 |
| Chromosome | 17 |
| Deletion Start | 31,144,245 |
| Deletion End | 31,141,176 |
| Deletion Size | 3,070 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,143,967 | 31,143,985 | GGGAAGCTCACCCAGAATC |
| TUP | 31,143,934 | 31,143,960 | TCAGAAGGGCTATGTCTACAGGTGTCA |
| TUR | 31,143,908 | 31,143,928 | AGGCCCTCCTGTCCTCCTAAC |
| TDF | 31,141,281 | 31,141,299 | GGGCAGCCACACTTCTGAG |
| TDP | 31,141,254 | 31,141,277 | AGGCACTCATGCCTCCTTGGCGTC |
| TDR | 31,141,191 | 31,141,210 | GCTCGGCAGTAGCAACTCTC |
PCR Primers
| Primer | Sequence |
| SU | CTATTCCAGTTGCATACGTG |
| SD | TCAGAGGGATGGAAGAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12557A-B1 | VGB6 | C57BL/6NTac | |
| 12557A-F1 | VGB6 | C57BL/6NTac | |
