| Gene | Pdgfrb |
| Chromosome | 18 |
| Deletion Start | 61,060,153 |
| Deletion End | 61,083,478 |
| Deletion Size | 23,326 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,060,564 | 61,060,585 | CTGCCCTACTGACCACTGTTTG |
| TUP | 61,060,587 | 61,060,608 | TGGCCCTGCCTACTGGCTGCAT |
| TUR | 61,060,630 | 61,060,649 | CACCACACCCTCATGTTCTC |
| TDF | 61,083,209 | 61,083,230 | CCCTTCCTCTAGTTCCACCTTG |
| TDP | 61,083,238 | 61,083,263 | TCAACACTTCCTCCACCATCTCCTGC |
| TDR | 61,083,282 | 61,083,301 | GCTTGCTGTGGCTCTTCTTG |
PCR Primers
| Primer | Sequence |
| SU | GTATTGGAACTGTCACCGGG |
| SD | GTTACAGTTTGGCCAGGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12560A-E2 | VGB6 | C57BL/6NTac | |
| 12560A-G1 | VGB6 | C57BL/6NTac | |
