| Gene | Bdnf |
| Chromosome | 2 |
| Deletion Start | 109,723,406 |
| Deletion End | 109,724,161 |
| Deletion Size | 756 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,723,610 | 109,723,630 | GAAGAGCTGCTGGATGAGGAC |
| TUP | 109,723,631 | 109,723,655 | CAGAAGGTTCGGCCCAACGAAGAAA |
| TUR | 109,723,699 | 109,723,719 | GGCTCCAAAGGCACTTGACTG |
| TDF | 109,723,894 | 109,723,916 | GACGGTCACAGTCCTAGAGAAAG |
| TDP | 109,723,917 | 109,723,940 | TCCCGGTATCCAAAGGCCAACTGA |
| TDR | 109,724,020 | 109,724,040 | GTAGTTCGGCATTGCGAGTTC |
PCR Primers
| Primer | Sequence |
| SU | ACAGTGTGTTCACTTGCTTAG |
| SD | TCTTGGCAACGGCAACAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 147 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12561A-G1 | VGB6 | C57BL/6NTac | |
| 12561D-H2 | VGB6 | C57BL/6NTac | |
| 12561E-E2 | VGB6 | C57BL/6NTac | |
| 12561E-F4 | VGB6 | C57BL/6NTac | |
| 12561E-F1 | VGB6 | C57BL/6NTac | |
