| Gene | Scube3 |
| Chromosome | 17 |
| Deletion Start | 28,142,529 |
| Deletion End | 28,171,365 |
| Deletion Size | 28,837 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,142,969 | 28,142,991 | TCTGCTATACGCTTCGTAACCTC |
| TUP | 28,142,996 | 28,143,017 | CCTGCCCGCATGAGGCAGAGTC |
| TUR | 28,143,031 | 28,143,055 | TTCGACTTGATAAGACGGTAACTTC |
| TDF | 28,170,577 | 28,170,602 | GGAGAGTTGGCTCAGTAGTTAAGAAC |
| TDP | 28,170,606 | 28,170,631 | TGTTACTTCTTGCAGAGGAACTGGGC |
| TDR | 28,170,653 | 28,170,677 | GGAGTTATGGATGGTTATGAGTCAC |
PCR Primers
| Primer | Sequence |
| SU | AGACGAGAAAGCAAGGGAGG |
| SD | GCTGCTATGTCCAAGGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12567A-D4 | VGB6 | C57BL/6NTac | |
| 12567A-D3 | VGB6 | C57BL/6NTac | |
