| Gene | Thpo |
| Chromosome | 16 |
| Deletion Start | 20,729,073 |
| Deletion End | 20,725,326 |
| Deletion Size | 3,748 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,728,427 | 20,728,450 | GACCCTTTGTCTATCCCTGTTCTG |
| TUP | 20,728,402 | 20,728,426 | CTGCCTGCTGTGGACTTTAGCCTGG |
| TUR | 20,728,360 | 20,728,381 | CCTGAGTCCAGGAGCCTCTTAC |
| TDF | 20,725,636 | 20,725,653 | TCGCCCGGAGCTTTCAAC |
| TDP | 20,725,610 | 20,725,633 | AGGCTCCCTGGCATTCAACCTCCA |
| TDR | 20,725,578 | 20,725,598 | GAGCAAGGCTTGGAGAAGGAG |
PCR Primers
| Primer | Sequence |
| SU | GGGCAACAATTGGTAGATGG |
| SD | TACAGTGAAGGGAAGGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12573A-D4 | VGB6 | C57BL/6NTac | |
| 12573A-E7 | VGB6 | C57BL/6NTac | |
| 12573A-F8 | VGB6 | C57BL/6NTac | |
| 12573A-G9 | VGB6 | C57BL/6NTac | |
| 12573A-H6 | VGB6 | C57BL/6NTac | |
