| Gene | Zfp637 |
| Chromosome | 6 |
| Deletion Start | 117,843,326 |
| Deletion End | 117,845,873 |
| Deletion Size | 2,548 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,844,053 | 117,844,075 | GAGATAGGGCCACTGTGATGAAG |
| TUP | 117,844,080 | 117,844,104 | TGCAGTGTTAGCCTAAGGGCTTCCC |
| TUR | 117,844,114 | 117,844,132 | GCCAAGCAAAGGCGAGAAC |
| TDF | 117,844,959 | 117,844,980 | CTCCCTGTTCTTCTCCCTGTTC |
| TDP | 117,844,988 | 117,845,015 | TGGTGCATCTTACATGACTTCCCTCTCG |
| TDR | 117,845,029 | 117,845,052 | TCATACTTGTGGGCTTCTGTCATC |
PCR Primers
| Primer | Sequence |
| SU | TCCAGTCCTGCTATCTTTAGCC |
| SD | GATTCCACCCAGTGGTCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12574A-B8 | VGB6 | C57BL/6NTac | |
| 12574A-D7 | VGB6 | C57BL/6NTac | |
| 12574A-E7 | VGB6 | C57BL/6NTac | |
| 12574A-F9 | VGB6 | C57BL/6NTac | |
| 12574A-B3 | VGB6 | C57BL/6NTac | |
