| Gene | Gpr149 |
| Chromosome | 3 |
| Deletion Start | 62,604,573 |
| Deletion End | 62,594,812 |
| Deletion Size | 9,762 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,604,146 | 62,604,168 | AGGACGGTGGAGAGTCAGTCAAG |
| TUP | 62,604,118 | 62,604,144 | TCCTGGCGATTAGGACAGGTACTTGGT |
| TUR | 62,604,095 | 62,604,117 | ACTGCCCAAACGGTTAAAGTCAC |
| TDF | 62,595,073 | 62,595,097 | ACCGCAGTGTCTTCAATACCATCAC |
| TDP | 62,595,045 | 62,595,067 | AAATCAGCACCACGCCACCGCTG |
| TDR | 62,594,973 | 62,594,995 | TTTCATGGCCTGTTTGGATTCTG |
PCR Primers
| Primer | Sequence |
| SU | TGTGCAATCTGCCTGAGAAG |
| SD | TCCATCTATATATACTGACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12580C-B10 | VGB6 | C57BL/6NTac | |
| 12580C-G5 | VGB6 | C57BL/6NTac | |
| 12580C-G9 | VGB6 | C57BL/6NTac | |
