| Gene | Cbln2 |
| Chromosome | 18 |
| Deletion Start | 86,713,072 |
| Deletion End | 86,716,852 |
| Deletion Size | 3,781 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,713,305 | 86,713,324 | TTCTTCCCTGGGCATTTCTG |
| TUP | 86,713,325 | 86,713,344 | TGCGCTCAGGCAGTGCCAAG |
| TUR | 86,713,371 | 86,713,391 | ATCTCTGACGGCTCGTGGTTG |
| TDF | 86,716,549 | 86,716,572 | GTCTCTAACCTGCTGAGTCTTCTG |
| TDP | 86,716,579 | 86,716,606 | AGGTCAGCTTAATGCAGAATGGCTACCC |
| TDR | 86,716,638 | 86,716,656 | TGGCTGCTTCCCTGGTAAC |
PCR Primers
| Primer | Sequence |
| SU | ACCAGTGCTCCAGAGCTTTC |
| SD | GTTAGGTGGTCCTCTGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12582C-G9 | VGB6 | C57BL/6NTac | |
| 12582C-D6 | VGB6 | C57BL/6NTac | |
| 12582C-F12 | VGB6 | C57BL/6NTac | |
| 12582C-A1 | VGB6 | C57BL/6NTac | |
