| Gene | Angptl1 |
| Chromosome | 1 |
| Deletion Start | 156,844,609 |
| Deletion End | 156,860,621 |
| Deletion Size | 16,013 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 156,844,795 | 156,844,815 | TGTGTCAACACCAAAGGTCAG |
| TUP | 156,844,818 | 156,844,846 | TGCAGGCACTATTAAGGACATGATCACCA |
| TUR | 156,844,869 | 156,844,886 | TGCCTAGACAGCACATCC |
| TDF | 156,860,339 | 156,860,358 | AGCACATACGTGTGGCAGTC |
| TDP | 156,860,359 | 156,860,383 | TGCAAGTGTCATGGCACAGAAGGAC |
| TDR | 156,860,389 | 156,860,413 | TGCAGGAGAAATAGAGACAGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | TTTGCAGTAAGACTTAACTCTG |
| SD | TGAACATGTATTTGGATTTGATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12583A-B5 | VGB6 | C57BL/6NTac | |
| 12583C-A3 | VGB6 | C57BL/6NTac | |
