| Gene | C1qtnf7 |
| Chromosome | 5 |
| Deletion Start | 43,609,064 |
| Deletion End | 43,616,325 |
| Deletion Size | 7,262 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,609,553 | 43,609,573 | TGTGGGAGATGAGCACAGATG |
| TUP | 43,609,575 | 43,609,597 | ACAGGTGCTGTGCATGACCGCTG |
| TUR | 43,609,621 | 43,609,641 | TGTTGTCCAGTGGTTCCAGTC |
| TDF | 43,615,984 | 43,616,005 | GGCCAATAAGCACCTAGCAATC |
| TDP | 43,616,009 | 43,616,034 | CTGGTGCACAATGGGCAGTACCGGAT |
| TDR | 43,616,080 | 43,616,100 | TGCAGGTAGATGACTGTGGAC |
PCR Primers
| Primer | Sequence |
| SU | CATAATGGCAACTGTGCTGG |
| SD | GAGAATTGTCAACAAATTTCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 117 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12585C-D10 | VGB6 | C57BL/6NTac | |
| 12585C-E6 | VGB6 | C57BL/6NTac | |
| 12585C-A2 | VGB6 | C57BL/6NTac | |
| 12585C-A12 | VGB6 | C57BL/6NTac | |
