| Gene | C1qtnf9 |
| Chromosome | 14 |
| Deletion Start | 60,772,300 |
| Deletion End | 60,780,024 |
| Deletion Size | 7,725 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,772,434 | 60,772,453 | TGGTGCCAAGGGTGACAAAG |
| TUP | 60,772,455 | 60,772,480 | AGACGCAGGTACTTGACACTTGCTGC |
| TUR | 60,772,494 | 60,772,516 | GTGGGTGACTGAAGGGATAGAAG |
| TDF | 60,779,841 | 60,779,862 | CCTGCACACCAAGGACAGTTAC |
| TDP | 60,779,864 | 60,779,886 | TGAGCTCTGAGGACCAGGCGTCT |
| TDR | 60,779,920 | 60,779,940 | TGTCACCTGCATCCACACTTC |
PCR Primers
| Primer | Sequence |
| SU | CTTCTTCTAGTCCAGGTTGG |
| SD | CTGTAGGACTAACTTCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12588A-C6 | VGB6 | C57BL/6NTac | |
| 12588A-C12 | VGB6 | C57BL/6NTac | |
| 12588A-E11 | VGB6 | C57BL/6NTac | |
| 12588A-H11 | VGB6 | C57BL/6NTac | |
| 12588A-G1 | VGB6 | C57BL/6NTac | |
