| Gene | Cdcp1 |
| Chromosome | 9 |
| Deletion Start | 123,190,119 |
| Deletion End | 123,173,432 |
| Deletion Size | 16,688 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,189,759 | 123,189,779 | CAGCTCCCTAAGATGGCTCTC |
| TUP | 123,189,721 | 123,189,741 | CCGGCCCATGTGCTTCCCAGA |
| TUR | 123,189,703 | 123,189,720 | TCTGGTCTGCCTGGGTTC |
| TDF | 123,173,938 | 123,173,959 | TCCTCATTCTGTTGTGCCATTC |
| TDP | 123,173,906 | 123,173,934 | ACAAACAGGAAGAAGGTGGATAAGGGTCC |
| TDR | 123,173,810 | 123,173,832 | GCATACACATGGGAGTCATTGTC |
PCR Primers
| Primer | Sequence |
| SU | CTGATTTCCTACTCCCACTC |
| SD | GTGAGAGCAGAGACCTCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 150 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12591B-B10 | VGB6 | C57BL/6NTac | |
| 12591D-B3 | VGB6 | C57BL/6NTac | |
| 12591D-B9 | VGB6 | C57BL/6NTac | |
