| Gene | Try4 |
| Chromosome | 6 |
| Deletion Start | 41,302,288 |
| Deletion End | 41,305,488 |
| Deletion Size | 3,201 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,303,096 | 41,303,115 | TTTGCAGTGCTGTGTAGTGC |
| TUP | 41,303,119 | 41,303,145 | ACTGTGTTGAACAAGCTGTTCCATGCC |
| TUR | 41,303,205 | 41,303,225 | AGCCCTCAGCTCTCAACTAAC |
| TDF | 41,304,841 | 41,304,862 | CTTGAAGGATGCTCCATGGTCT |
| TDP | | | AGACTTTCTCCACTGGA |
| TDR | 41,304,896 | 41,304,920 | AAGAGAGAATGTGATTCAAACCAGG |
PCR Primers
| Primer | Sequence |
| SU | AGGTCCTGCATATCTGCCAC |
| SD | GTTTTCTTTGAAACTGCTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 130 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12597A-A4 | VGB6 | C57BL/6NTac | |
| 12597A-D6 | VGB6 | C57BL/6NTac | |
| 12597A-F4 | VGB6 | C57BL/6NTac | |
| 12597A-C7 | VGB6 | C57BL/6NTac | |
