| Gene | Tle2 |
| Chromosome | 10 |
| Deletion Start | 81,575,671 |
| Deletion End | 81,590,393 |
| Deletion Size | 14,723 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,576,432 | 81,576,450 | AGCCCATCCGTGATCCATC |
| TUP | 81,576,451 | 81,576,474 | TTGTGGGTTCCCTGTCTGGGTCTC |
| TUR | 81,576,496 | 81,576,517 | GGTACGAGTATCAGGGCAAGAC |
| TDF | 81,589,517 | 81,589,536 | GGAGGCCAGCTCGACATTTC |
| TDP | 81,589,540 | 81,589,568 | TCCTAGACACTTCCTGTTACAGAGTCCCA |
| TDR | 81,589,571 | 81,589,591 | GGTACACAGAGGGACCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | CTGGCTGCTCTTTCTTTGCG |
| SD | TGGTTCAGTTGGTAAAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12600A-A4 | VGB6 | C57BL/6NTac | |
| 12600A-F10 | VGB6 | C57BL/6NTac | |
