| Gene | Adam26a |
| Chromosome | 8 |
| Deletion Start | 43,570,448 |
| Deletion End | 43,568,343 |
| Deletion Size | 2,106 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,570,115 | 43,570,137 | AGACCTAGAGTCCCCGGTCATTG |
| TUP | 43,570,083 | 43,570,110 | CACCTGTTTTGGAAGTTTGCAAGGCACA |
| TUR | 43,569,990 | 43,570,011 | TGAATCCCCACTGTCCATTTTG |
| TDF | 43,568,831 | 43,568,855 | AGCTCTACATATAGAACATGCCAAA |
| TDP | 43,568,797 | 43,568,826 | TGATGTACTCTGTGGGCAAATTCAGTGTGA |
| TDR | 43,568,708 | 43,568,732 | GATAGTCCATAGTCCAGCAGGTGAT |
PCR Primers
| Primer | Sequence |
| SU | TGTTGTGCATAGGGTGAAATTAG |
| SD | AAAGCAAAGTAGAGGATGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 148 bp | + | + | - |
| TDF | TDR | 148 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12601A-G1 | VGB6 | C57BL/6NTac | |
| 12601B-F11 | VGB6 | C57BL/6NTac | |
