| Gene | St8sia3 |
| Chromosome | 18 |
| Deletion Start | 64,265,475 |
| Deletion End | 64,271,885 |
| Deletion Size | 6,411 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,265,957 | 64,265,979 | AGAGATGGACAAGGCTTGAGGTG |
| TUP | 64,265,980 | 64,266,002 | AGCGACCTGGCTGAGCACTAGCG |
| TUR | 64,266,049 | 64,266,068 | GAAGGGATGCGAGAGCAGTC |
| TDF | 64,271,367 | 64,271,386 | CGCATACTGAAGCTGGGAAG |
| TDP | 64,271,387 | 64,271,414 | TGTATCTAGCGGTGAAACCCAAACAGTC |
| TDR | 64,271,428 | 64,271,449 | AGTCAAGAAGCAGGGCAAGGAG |
PCR Primers
| Primer | Sequence |
| SU | CCATCTTCCATTTCAGCTCC |
| SD | CAGAGCTCCCTGGTTGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12602A-B3 | VGB6 | C57BL/6NTac | |
| 12602A-F1 | VGB6 | C57BL/6NTac | |
| 12602A-F4 | VGB6 | C57BL/6NTac | |
