| Gene | Rtkn |
| Chromosome | 6 |
| Deletion Start | 83,138,178 |
| Deletion End | 83,152,254 |
| Deletion Size | 14,077 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,138,465 | 83,138,487 | CCTGTTTGACACGGACAAAGTTC |
| TUP | 83,138,488 | 83,138,511 | TGGGTTTGACTCTCAGCAGCCTGG |
| TUR | 83,138,513 | 83,138,532 | GCAACGCAGTTCATGCAGTG |
| TDF | 83,151,875 | 83,151,893 | TGCTGCCACAGCCATTGAG |
| TDP | 83,151,895 | 83,151,918 | CGCTAGACGACATCGCAGCTGTGA |
| TDR | 83,151,976 | 83,151,999 | AGGCAGGCTGGTCTGTAAACATTG |
PCR Primers
| Primer | Sequence |
| SU | TTCTTTCCTCCTCTGCTTGG |
| SD | GAGAGGTGGGTTCAGAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12604B-C4 | VGB6 | C57BL/6NTac | |
| 12604B-E1 | VGB6 | C57BL/6NTac | |
