| Gene | Ccr10 |
| Chromosome | 11 |
| Deletion Start | 101,175,420 |
| Deletion End | 101,173,516 |
| Deletion Size | 1,905 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,174,695 | 101,174,716 | GTCTTACACGGTCCCTCTTCTC |
| TUP | 101,174,669 | 101,174,692 | TTTGATGCCACCAGGTCTCCTGGG |
| TUR | 101,174,647 | 101,174,668 | TCATCGTACCCGGAGTAAAGTC |
| TDF | 101,174,331 | 101,174,350 | GCCTCTACTCGGCCTCTTTC |
| TDP | 101,174,307 | 101,174,330 | CACGCTGGCTTCCTCTTCCTAGCC |
| TDR | 101,174,286 | 101,174,305 | ATAGCGGTCGGCGCTGATAC |
PCR Primers
| Primer | Sequence |
| SU | TTAACCCAAGGCTGACAAGG |
| SD | CTCCTCACCTGGCAGCTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12608A-E4 | VGB6 | C57BL/6NTac | |
| 12608A-E12 | VGB6 | C57BL/6NTac | |
