| Gene | Dgcr6 |
| Chromosome | 16 |
| Deletion Start | 18,065,162 |
| Deletion End | 18,071,034 |
| Deletion Size | 5,873 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,065,483 | 18,065,502 | GCCCTCAGTGACACTCTTTC |
| TUP | 18,065,503 | 18,065,532 | TCCAATAAAGCCACACCTCCTAATCCTTCC |
| TUR | 18,065,572 | 18,065,595 | ATGCAAATGACCCCATAGCCTCAG |
| TDF | 18,070,059 | 18,070,078 | AGCAGGAACCACCCTTACTC |
| TDP | 18,070,084 | 18,070,109 | ATGGCAGGCCATGGAACATCGGATCC |
| TDR | 18,070,125 | 18,070,148 | CCAGGACAATCTTTCGGTCCATAG |
PCR Primers
| Primer | Sequence |
| SU | CACCATAATTTGGTATACTGTC |
| SD | CTCTGGCTTCTTCCATCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12609A-B4 | VGB6 | C57BL/6NTac | |
| 12609A-H3 | VGB6 | C57BL/6NTac | |
| 12609A-H2 | VGB6 | C57BL/6NTac | |
| 12609A-F7 | VGB6 | C57BL/6NTac | |
| 12609A-D8 | VGB6 | C57BL/6NTac | |
| 12609A-H10 | VGB6 | C57BL/6NTac | |
