| Gene | Rasd1 |
| Chromosome | 11 |
| Deletion Start | 59,964,802 |
| Deletion End | 59,963,710 |
| Deletion Size | 1,093 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,964,757 | 59,964,774 | GCCCAAGCGACTCTGAAC |
| TUP | 59,964,734 | 59,964,756 | TGAGTATCCCGGCCAAGAACTGC |
| TUR | 59,964,705 | 59,964,724 | TTGGATGAGCCGAGGATGAC |
| TDF | 59,964,172 | 59,964,193 | GAAGTAGAGCAGCGGGAGATTG |
| TDP | 59,964,147 | 59,964,168 | AGCTGGTGGGTGACGACCCTCA |
| TDR | 59,964,076 | 59,964,095 | CAAAGAGCGCACGGAACATC |
PCR Primers
| Primer | Sequence |
| SU | AGTGAATCAAAGGCACAGCC |
| SD | AGCCAAAGTTTCCAGTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12610A-A8 | VGB6 | C57BL/6NTac | |
| 12610A-B10 | VGB6 | C57BL/6NTac | |
| 12610A-C4 | VGB6 | C57BL/6NTac | |
| 12610A-D3 | VGB6 | C57BL/6NTac | |
