| Gene | Slc27a5 |
| Chromosome | 7 |
| Deletion Start | 12,998,059 |
| Deletion End | 12,988,471 |
| Deletion Size | 9,589 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,997,385 | 12,997,403 | GGCCAGTGTGCTGATTGTG |
| TUP | 12,997,360 | 12,997,383 | ATCCAGGTGAGTGCCCTAGAGGCA |
| TUR | 12,997,331 | 12,997,350 | CCCTTGCTTCTGTCCCTTTC |
| TDF | 12,988,812 | 12,988,831 | AAACTGGCTCCTGGGAAGAC |
| TDP | 12,988,781 | 12,988,808 | TGATGGGCAGAAGCTATACCAGCATGTC |
| TDR | 12,988,735 | 12,988,757 | GGATACGGATGAAATGAGGTGTG |
PCR Primers
| Primer | Sequence |
| SU | TGGCTAGAGTCAGGCAGGTC |
| SD | CTGACCCAAGCTTGTCCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12615A-A1 | VGB6 | C57BL/6NTac | |
| 12615A-D2 | VGB6 | C57BL/6NTac | |
| 12615A-H5 | VGB6 | C57BL/6NTac | |
