| Gene | Foxf2 |
| Chromosome | 13 |
| Deletion Start | 31,626,083 |
| Deletion End | 31,630,725 |
| Deletion Size | 4,643 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,626,783 | 31,626,800 | TGGGTTGCCACGGTCAAG |
| TUP | 31,626,802 | 31,626,827 | CGGTTATGGTGGCCTCGACATGATGC |
| TUR | 31,626,835 | 31,626,856 | AGCACCCGCCCCTGTATCATAG |
| TDF | 31,630,604 | 31,630,622 | CACCAGAGCGTGTGCCAAG |
| TDP | 31,630,631 | 31,630,658 | CCCTGTGTTATGTGAATGGACAGAGGCC |
| TDR | 31,630,680 | 31,630,703 | GGAGAGGAGAAGAAGGCAGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCTGAAAGAAGACGACGG |
| SD | GCCAGGGAACTAGAGAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12618A-B7 | VGB6 | C57BL/6NTac | |
| 12618A-E6 | VGB6 | C57BL/6NTac | |
| 12618A-D2 | VGB6 | C57BL/6NTac | |
| 12618A-F8 | VGB6 | C57BL/6NTac | |
