| Gene | Fbxl8 |
| Chromosome | 8 |
| Deletion Start | 105,267,017 |
| Deletion End | 105,269,080 |
| Deletion Size | 2,064 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,267,028 | 105,267,049 | CGACAATCTGCCAGAGGAAGTG |
| TUP | 105,267,050 | 105,267,071 | TTGGCGCTCATCTTCCGTGACC |
| TUR | 105,267,083 | 105,267,101 | TGGTGGCTACAGCAAGGTC |
| TDF | 105,268,642 | 105,268,660 | TTCCAGATGAGGCCGTGAC |
| TDP | 105,268,662 | 105,268,686 | CGCATCCTGCAACCAGCAGTACCAG |
| TDR | 105,268,690 | 105,268,709 | GAGGTTGAGACGCAGCACAG |
PCR Primers
| Primer | Sequence |
| SU | CCTGGGATCTCTGCCTTCTC |
| SD | GCCTTTGTGTATTTCCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12621B-C8 | VGB6 | C57BL/6NTac | |
| 12621B-G3 | VGB6 | C57BL/6NTac | |
| 12621B-H5 | VGB6 | C57BL/6NTac | |
| 12621B-G12 | VGB6 | C57BL/6NTac | |
