| Gene | Akirin2 |
| Chromosome | 4 |
| Deletion Start | 34,551,048 |
| Deletion End | 34,566,261 |
| Deletion Size | 15,214 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,551,581 | 34,551,604 | GGTTGCAGCTATTAACTCAGTGAC |
| TUP | 34,551,612 | 34,551,634 | TTTGAGGCGAGAAGGCAGCCCCT |
| TUR | 34,551,636 | 34,551,660 | AAAACCAGCCAAGTCTAAAATGGAG |
| TDF | 34,565,458 | 34,565,478 | CACCCCTAGTGGCACACTTCT |
| TDP | | | ACCAATGAGGCCACAC |
| TDR | 34,565,517 | 34,565,538 | GCAAGTCAGCAGGGAGTATAAC |
PCR Primers
| Primer | Sequence |
| SU | GAGTGCATTGCTTAGCTCTG |
| SD | AACCAGTGAAGGGCATGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12624A-H6 | VGB6 | C57BL/6NTac | |
| 12624A-G4 | VGB6 | C57BL/6NTac | |
| 12624A-D11 | VGB6 | C57BL/6NTac | |
| 12624A-H4 | VGB6 | C57BL/6NTac | |
