| Gene | Sec11c |
| Chromosome | 18 |
| Deletion Start | 65,800,628 |
| Deletion End | 65,817,513 |
| Deletion Size | 16,886 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,801,015 | 65,801,034 | TTGAGGGCCACTCAGTGAAG |
| TUP | 65,801,039 | 65,801,060 | AGCAGCTTTCCCTGTGGCGCTC |
| TUR | 65,801,085 | 65,801,105 | GGTCAGCCTCTTAGCCTCTTG |
| TDF | 65,817,211 | 65,817,232 | AGGCAACAGGGAGCGTACAAAG |
| TDP | 65,817,234 | 65,817,257 | AGTAAGCGCCGCATTAGCCGTGTC |
| TDR | 65,817,297 | 65,817,318 | AAATTAGCCGTTCAATGCACAC |
PCR Primers
| Primer | Sequence |
| SU | GCGCCTGACCAGAGTCTTAG |
| SD | AGCTTGTCTCCCTCAGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12626B-A7 | VGB6 | C57BL/6NTac | |
| 12626B-E8 | VGB6 | C57BL/6NTac | |
| 12626B-H2 | VGB6 | C57BL/6NTac | |
