| Gene | Susd3 |
| Chromosome | 13 |
| Deletion Start | 49,248,129 |
| Deletion End | 49,231,126 |
| Deletion Size | 17,004 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,247,331 | 49,247,352 | GGAGGAAGCTGAGGATCTGTAG |
| TUP | 49,247,303 | 49,247,328 | ACATTGCCCTCACCCTAGAGCAGTGC |
| TUR | 49,247,279 | 49,247,298 | TCGCAGCATTATGACGGTTG |
| TDF | 49,231,208 | 49,231,226 | GCCAGGCACCTACGTGATG |
| TDP | 49,231,184 | 49,231,206 | TCCACGCGTTGAACTCTGCAGGG |
| TDR | 49,231,136 | 49,231,157 | TCACCCTGGAAGGTACACTTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGGCTGTCACTCTCCAAGG |
| SD | CCCAGAAGATGATAAGGTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12627A-G9 | VGB6 | C57BL/6NTac | |
| 12627A-G8 | VGB6 | C57BL/6NTac | |
| 12627A-F3 | VGB6 | C57BL/6NTac | |
| 12627A-E9 | VGB6 | C57BL/6NTac | |
| 12627A-G12 | VGB6 | C57BL/6NTac | |
