| Gene | Asrgl1 |
| Chromosome | 19 |
| Deletion Start | 9,135,551 |
| Deletion End | 9,112,932 |
| Deletion Size | 22,620 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,134,993 | 9,135,013 | GACACTCGCCTCTTCCTGATG |
| TUP | 9,134,954 | 9,134,982 | ATCGATGTGAGTCTCGTGGTGGTAGTCCA |
| TUR | 9,134,919 | 9,134,938 | TCCGGTTGGCTGAGATGTTG |
| TDF | 9,113,310 | 9,113,333 | TCCAGTGAAGCCCATTCATATCTC |
| TDP | 9,113,284 | 9,113,308 | CAGTGTCCCAAAGCAGTGGCATAGC |
| TDR | 9,113,249 | 9,113,269 | TTTCAGAGGCTCCCATGTTTG |
PCR Primers
| Primer | Sequence |
| SU | AGGAGAGGAGCTGGGATTTC |
| SD | TGAGCTTCACCTGGACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12628A-E1 | VGB6 | C57BL/6NTac | |
| 12628A-G2 | VGB6 | C57BL/6NTac | |
| 12628A-D1 | VGB6 | C57BL/6NTac | |
| 12628A-F8 | VGB6 | C57BL/6NTac | |
