| Gene | Lyzl1 |
| Chromosome | 18 |
| Deletion Start | 4,168,525 |
| Deletion End | 4,182,025 |
| Deletion Size | 13,501 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,169,122 | 4,169,143 | TTCCTCCTCATTTAGGGCTCTG |
| TUP | 4,169,152 | 4,169,181 | ACTATGAGAGCCACTACAACACTACAGCCG |
| TUR | 4,169,183 | 4,169,202 | TTCCATCCTCCAGGACATTC |
| TDF | 4,181,087 | 4,181,106 | TGCTGACCCTCACATCTTTC |
| TDP | 4,181,110 | 4,181,136 | ACAGCCTTGATCACTGATGACCTCACA |
| TDR | 4,181,137 | 4,181,155 | TGGCACAGAGAATGGCATC |
PCR Primers
| Primer | Sequence |
| SU | CCAGTCTTATTCCTGCCCTG |
| SD | GGAATTGTCAAACTGGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12631A-H11 | VGB6 | C57BL/6NTac | |
| 12631A-A7 | VGB6 | C57BL/6NTac | |
