| Gene | Eepd1 |
| Chromosome | 9 |
| Deletion Start | 25,482,445 |
| Deletion End | 25,483,314 |
| Deletion Size | 870 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,482,484 | 25,482,504 | CCCTCGGACCTGTCACATAAC |
| TUP | 25,482,514 | 25,482,535 | AGCGCTGCCTGCAACTTCAGCA |
| TUR | 25,482,570 | 25,482,588 | TTCCTCCTCAGTGGCAGTG |
| TDF | 25,483,077 | 25,483,096 | CTTCACTGCCAAGCCTCATC |
| TDP | 25,483,098 | 25,483,121 | CAGTCCCACATCTCTGAGCCTGCA |
| TDR | 25,483,124 | 25,483,141 | GCAGGTCCAAGTCCTCAC |
PCR Primers
| Primer | Sequence |
| SU | CAGCGTTGAGGCTAGTGGAG |
| SD | TACATGCAAGACCGTCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12632A-A5 | VGB6 | C57BL/6NTac | |
| 12632A-E2 | VGB6 | C57BL/6NTac | |
| 12632A-F1 | VGB6 | C57BL/6NTac | |
| 12632A-F6 | VGB6 | C57BL/6NTac | |
