| Gene | Ttc33 |
| Chromosome | 15 |
| Deletion Start | 5,189,586 |
| Deletion End | 5,217,587 |
| Deletion Size | 28,002 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,190,322 | 5,190,343 | GCCCTTCCACATCAAACATCAG |
| TUP | 5,190,353 | 5,190,377 | TATGTTCCACAGGCTCGCCCACTGG |
| TUR | 5,190,381 | 5,190,402 | TGAGAACACATATCCACCAGAC |
| TDF | 5,217,194 | 5,217,215 | CCCTGAACTCTGGAAGGAAGAC |
| TDP | 5,217,221 | 5,217,244 | TTGGGCCAGAAAGCTCCAAGAACA |
| TDR | 5,217,248 | 5,217,269 | TTCAATCCTCTGGGCTACTTTC |
PCR Primers
| Primer | Sequence |
| SU | TCTCACCCACATCTGATGTG |
| SD | TCGGCACTGTAGTAACACGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12633A-G6 | VGB6 | C57BL/6NTac | |
| 12633A-E3 | VGB6 | C57BL/6NTac | |
| 12633A-D3 | VGB6 | C57BL/6NTac | |
