| Gene | Sdccag3 |
| Chromosome | 2 |
| Deletion Start | 26,388,958 |
| Deletion End | 26,383,503 |
| Deletion Size | 5,456 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,388,681 | 26,388,699 | CCGCATCCAGCCTCAGAAG |
| TUP | 26,388,653 | 26,388,680 | ACAGCTTTCTAGTCCCAGACTCAGTGGG |
| TUR | 26,388,615 | 26,388,635 | CGTTTGTCCAGGGAGGTGAAG |
| TDF | 26,384,378 | 26,384,399 | CCCTGGCTTCTCTGACAGTATG |
| TDP | 26,384,350 | 26,384,375 | TAGACCGGGTTAGCCTCAGACTCAGA |
| TDR | 26,384,328 | 26,384,349 | TGGGAAATGGAAGCAGGAAATC |
PCR Primers
| Primer | Sequence |
| SU | GCTTCTGCGGTCACTTCC |
| SD | TTCCAAAGCTGACAGCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12635A-B8 | VGB6 | C57BL/6NTac | |
| 12635A-E12 | VGB6 | C57BL/6NTac | |
| 12635A-F7 | VGB6 | C57BL/6NTac | |
| 12635A-F10 | VGB6 | C57BL/6NTac | |
