| Gene | Nrn1 |
| Chromosome | 13 |
| Deletion Start | 36,734,287 |
| Deletion End | 36,726,595 |
| Deletion Size | 7,693 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,734,229 | 36,734,249 | CGCGGTGCAAATAGGTAAGTG |
| TUP | 36,734,207 | 36,734,227 | CCGCCCGGTACGGCTTGGTAG |
| TUR | 36,734,127 | 36,734,146 | GCAGGGTTTTCTGCAACCAG |
| TDF | 36,726,931 | 36,726,954 | GAAGGTTAGAAAGGACGCAGTCAC |
| TDP | 36,726,900 | 36,726,922 | CAGACCTAGGGTCCTCCCGCACC |
| TDR | 36,726,834 | 36,726,857 | AGCTGTGGAAATCCTCCCAGTATC |
PCR Primers
| Primer | Sequence |
| SU | AGAGAGGCGAGATTTCGTTG |
| SD | CTTTCCCATTAGGAGGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12636A-B3 | VGB6 | C57BL/6NTac | |
| 12636A-G6 | VGB6 | C57BL/6NTac | |
| 12636A-G8 | VGB6 | C57BL/6NTac | |
| 12636A-F1 | VGB6 | C57BL/6NTac | |
