| Gene | Tspan11 |
| Chromosome | 6 |
| Deletion Start | 127,912,964 |
| Deletion End | 127,949,942 |
| Deletion Size | 36,979 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,913,333 | 127,913,351 | CCACCGTGGGTTTGGTCTC |
| TUP | 127,913,357 | 127,913,379 | TGCACTGAGGCAGGAATGGTCTC |
| TUR | 127,913,394 | 127,913,412 | CGCTGCTGTGGGAAACTTG |
| TDF | 127,948,970 | 127,948,993 | GGAAGAAGATGAGGAGGGACAAAC |
| TDP | 127,948,995 | 127,949,018 | TAACCTGGATGGCCTGAAGCCAGC |
| TDR | 127,949,038 | 127,949,060 | CATGAAGAGGAGTGTAGCCTGAG |
PCR Primers
| Primer | Sequence |
| SU | AACGTGTGAGTGTGCCTGAG |
| SD | CTGAGCTAACCTGGTCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12637A-A4 | VGB6 | C57BL/6NTac | |
| 12637C-D2 | VGB6 | C57BL/6NTac | |
| 12637C-E8 | VGB6 | C57BL/6NTac | |
