| Gene | Mrps18c |
| Chromosome | 5 |
| Deletion Start | 100,798,773 |
| Deletion End | 100,804,500 |
| Deletion Size | 5,728 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,799,089 | 100,799,109 | GACACTTGTTGGCTGTGGTTC |
| TUP | 100,799,110 | 100,799,134 | TCTTGCATCTGTTTCCATCCGCTGC |
| TUR | 100,799,159 | 100,799,183 | GAGACAGGAGCCTAGATAGCATAAC |
| TDF | 100,803,523 | 100,803,546 | ACACGGCGACAGCATATAGAAATC |
| TDP | 100,803,561 | 100,803,586 | ACTGTCTTGGGTCTAGCCCAGTGGCC |
| TDR | 100,803,641 | 100,803,661 | TGCTTGCAGAAGCCAGATGTG |
PCR Primers
| Primer | Sequence |
| SU | ACGGAGGTTCTCCAGGTTG |
| SD | CAGCCATTCACACAGGATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 139 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12639A-D8 | VGB6 | C57BL/6NTac | |
| 12639A-F6 | VGB6 | C57BL/6NTac | |
