| Gene | Paox |
| Chromosome | 7 |
| Deletion Start | 140,125,729 |
| Deletion End | 140,134,180 |
| Deletion Size | 8,452 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,126,130 | 140,126,150 | CCCAGGAGTTCCCGAAATCTG |
| TUP | 140,126,152 | 140,126,177 | TCTGACCAAGCCATGCCTTTAGGAAC |
| TUR | 140,126,252 | 140,126,271 | CCACCCTTTCGCAAGCATAC |
| TDF | 140,133,248 | 140,133,266 | TAGAGCAGTGGGTGCTGTC |
| TDP | 140,133,267 | 140,133,291 | CCCTTCCTCTGCCCACTACCTCTCC |
| TDR | 140,133,315 | 140,133,339 | TCAGTAAGTTAAGGGAGAGTGAGTG |
PCR Primers
| Primer | Sequence |
| SU | GGTTCTAGGCTCACACTGCC |
| SD | TTGAGAACTGGATGCACTATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 142 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12642A-E1 | VGB6 | C57BL/6NTac | |
| 12642A-G11 | VGB6 | C57BL/6NTac | |
| 12642A-E10 | VGB6 | C57BL/6NTac | |
| 12642A-C10 | VGB6 | C57BL/6NTac | |
