| Gene | 4931417G12Rik |
| Chromosome | 6 |
| Deletion Start | 113,598,040 |
| Deletion End | 113,597,474 |
| Deletion Size | 567 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,597,999 | 113,598,018 | CATGGACCCCACTGGATGAG |
| TUP | 113,597,977 | 113,597,998 | AGCTTCCAATGGCTGCGGCACA |
| TUR | 113,597,937 | 113,597,957 | GAGGCCCTAAAAGGGTGTTTG |
| TDF | 113,597,551 | 113,597,573 | ACGCTCAATCCTCTTGCTGTATG |
| TDP | 113,597,525 | 113,597,550 | CGACTTACAAGAAGTGCACCTTCGCC |
| TDR | 113,597,481 | 113,597,498 | TGGTGTGGAGGTCCAGTG |
PCR Primers
| Primer | Sequence |
| SU | TCATCATGAAGTCCAGCCAC |
| SD | TTGATTTGAGGAGGGAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12646A-E1 | VGB6 | C57BL/6NTac | |
| 12646A-C4 | VGB6 | C57BL/6NTac | |
| 12646A-E6 | VGB6 | C57BL/6NTac | |
| 12646A-G10 | VGB6 | C57BL/6NTac | |
| 12646A-H10 | VGB6 | C57BL/6NTac | |
