| Gene | Osgin1 |
| Chromosome | 8 |
| Deletion Start | 119,441,439 |
| Deletion End | 119,445,967 |
| Deletion Size | 4,529 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,441,969 | 119,441,987 | CTGGCTTAGGCAAGCACTC |
| TUP | 119,441,988 | 119,442,012 | TGCCTCTGAGCTACACTCCCACCTA |
| TUR | 119,442,040 | 119,442,057 | GGCCTGTCTGTGCAACAC |
| TDF | 119,444,985 | 119,445,005 | GGTGACATCGCCCACTACTAC |
| TDP | 119,445,006 | 119,445,032 | CGGGACTACGTGATCAAGAAAGGCCTA |
| TDR | 119,445,041 | 119,445,059 | CACGGCACCAGACACAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGCAGCCACCAAAGAAGTC |
| SD | CACAGACTCCTCAGGCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12649A-F10 | VGB6 | C57BL/6NTac | |
| 12649A-H1 | VGB6 | C57BL/6NTac | |
| 12649A-D12 | VGB6 | C57BL/6NTac | |
| 12649A-G2 | VGB6 | C57BL/6NTac | |
| 12649A-G5 | VGB6 | C57BL/6NTac | |
| 12649A-F9 | VGB6 | C57BL/6NTac | |
