| Gene | Macrod2 |
| Chromosome | 2 |
| Deletion Start | 140,400,976 |
| Deletion End | 140,420,199 |
| Deletion Size | 19,224 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,401,860 | 140,401,883 | GCAAAGTTTAGACAAACAGCACAC |
| TUP | 140,401,885 | 140,401,909 | TCAGAGCAGACTTGAGAGCAAGGGA |
| TUR | 140,401,935 | 140,401,958 | TCTAAGGGCACAATCTGTAAAGTG |
| TDF | 140,420,050 | 140,420,072 | CCTGAAGGCAAACAGACAAGAAG |
| TDP | 140,420,075 | 140,420,100 | TGGGAGACACATGCAGTTTTGTTGCG |
| TDR | 140,420,104 | 140,420,127 | TCCAGGAAGGTCAGTGTTAGTTAG |
PCR Primers
| Primer | Sequence |
| SU | TTCCTGAGCTCCGTGAATG |
| SD | TCTTTCAAGCTGACTGTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12650A-B1 | VGB6 | C57BL/6NTac | |
| 12650A-B5 | VGB6 | C57BL/6NTac | |
| 12650A-C6 | VGB6 | C57BL/6NTac | |
| 12650A-D6 | VGB6 | C57BL/6NTac | |
| 12650A-G2 | VGB6 | C57BL/6NTac | |
