12652   Tppp     Status: ES cell colonies screened / QC positives

GeneTppp
Chromosome13
Deletion Start74,021,146
Deletion End74,031,434
Deletion Size10,289
Genome Build38
MGI IDMGI:1920198
NCBI Gene ID72948
BAC ID452O8
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,021,17774,021,196CCAACAAGACACCACCCAAG
TUP74,021,20174,021,220CAGGAGACCCAGCCAGGGCT
TUR74,021,22674,021,245CTGACTCCAGTGACAACCTC
TDF74,030,83974,030,858AGGAGGCTGTACGTGAGGTG
TDP74,030,85974,030,882CACAGGCTCATTGAGGGACGAGCA
TDR74,030,88674,030,903CGTGACGCCAGAGATGAC

PCR Primers

PrimerSequence
SUGGCTCAGGTCTGAGGAACAG
SDAGTCCACTTTCCAGCTGTGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR69 bp++-
TDFTDR65 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12652A-F2VGB6C57BL/6NTac
12652A-F5VGB6C57BL/6NTac
12652A-G3VGB6C57BL/6NTac
12652A-H4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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