12653   Fam101a     Status: ES cell colonies screened / QC positives

GeneFam101a
Chromosome5
Deletion Start125,003,571
Deletion End125,011,630
Deletion Size8,060
Genome Build38
MGI IDMGI:1920371
NCBI Gene ID73121
BAC ID238L17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,003,783125,003,804GCATGGTCTCATCCCTCTTCTG
TUP125,003,811125,003,832ACGTGGAGTGGCACCCGACTCA
TUR125,003,840125,003,860CCCGAAACTTCCAGCCTACAG
TDF125,010,683125,010,702GTGGCACTGATGTCACTCAC
TDP125,010,703125,010,727TTGTCCTTCTGAAGAGGGAGCCACC
TDR125,010,747125,010,771ACCAGAAGAAAGCCTATTGAAAGTC

PCR Primers

PrimerSequence
SUACTTTGCAGTCCTCACCCTG
SDGCTAGTCTGGAATGCGTCTTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR78 bp++-
TDFTDR89 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12653A-H8VGB6C57BL/6NTac
12653A-C8VGB6C57BL/6NTac
12653A-A2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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