| Gene | 1700108M19Rik |
| Chromosome | 12 |
| Deletion Start | 36,212,688 |
| Deletion End | 36,208,509 |
| Deletion Size | 4,180 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,211,789 | 36,211,808 | GTATGCTGCCAGGCACAAAG |
| TUP | 36,211,768 | 36,211,788 | TGCTGGCCTAGCATGCCCAAA |
| TUR | 36,211,747 | 36,211,767 | AGTGCTGGAGATCAAACCAAC |
| TDF | 36,208,663 | 36,208,686 | CCCAGAAGATGCTGTGTTTGTAAG |
| TDP | 36,208,634 | 36,208,661 | TCCACGAAGAGATCCCTGATGACGTTCA |
| TDR | 36,208,611 | 36,208,632 | AACGGTGTTCCAGTTCATGCTG |
PCR Primers
| Primer | Sequence |
| SU | GAGTGCCACTATGACAGGAGG |
| SD | TATACGTGGGTAACTCATATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12655A-B7 | VGB6 | C57BL/6NTac | |
| 12655A-D4 | VGB6 | C57BL/6NTac | |
| 12655A-G1 | VGB6 | C57BL/6NTac | |
| 12655A-G11 | VGB6 | C57BL/6NTac | |
| 12655A-H6 | VGB6 | C57BL/6NTac | |
| 12655A-C3 | VGB6 | C57BL/6NTac | |
